多聚-D-赖氨酸 氢溴酸盐

Components

分子量 >30,000 的聚赖氨酸适用于促进细胞粘附到固体基质。

Analysis Note

This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Application

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine. 

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Poly-D-lysine hydrobromide is used for the following applications:
• Used in animal cell culture^[7]
• Rat cortical neuron cultures^[5]
• Cell culture and siRNA treatment^[1] 
• Primary cell cultures and adult neuronal network model^[4]
• Cortical astrocyte culture^[3]
• Immunofluorescence staining (HDF cells were plated onto poly-D lysine (P0899, Sigma-Aldrich) coated glass coverslips)^[6]
• NPC collection and culture conditions^[2]

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Caution

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

包装

1 g in poly bottle

50, 100, 500 mg in poly bottle

10 mg in glass bottle

性质

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