核糖核酸酶 A 来源于牛胰腺

Analysis Note

通过 E1%/280 确定的蛋白质。

Features and Benefits

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA

包装

1, 5 g in glass bottle

100, 500 mg in glass bottle

Preparation Note

Salt fractionated and chromatographically purified.

Application

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. RNase has been used as a tool for computer-aided drug design ^[1].

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His¹² and His¹¹⁹ and activated by potassium and sodium salts.

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